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M9480835.TXT
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1994-09-05
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Document 0835
DOCN M9480835
TI VIDAS vs ELISA in the serodiagnosis of HIV 1 + 2, rubella and
toxoplasma.
DT 9410
AU Araj GF; Bizri AR; Fattouh LG; Nassif RE; American Univ. Med. Ctr.,
Beirut, Lebanon.
SO Abstr Gen Meet Am Soc Microbiol. 1994;94:555 (abstract no. C-365).
Unique Identifier : AIDSLINE ASM94/94313110
AB Rapid, automated and self-contained instruments that minimize exposure
to potentially infectious blood samples are attractive addition to the
serodiagnostic laboratory. The performance of Vitek Immuno Diagnostic
Assay Systems (VIDAS, biomerieux Vitek Inc., Hazelwood, MO) was tried in
a developing country for the detection of antibodies to three infectious
diseases. Among 87 selected sera which were tested for anti-HIV 1 + 2,
20 and 13 (of the 20) were positive by ELISA (CLONATEC, Paris, France)
and VIDAS, respectively. The Western blot (Lia Tek HIV 1 + 2, Organon
Teknika, Boxtel, Holland) done on the 20 positive sera confirmed only
the 13 which were also positive by VIDAS. Concordant results were
obtained for 21 sera (12 positive) tested for anti-rubella IgG by ELISA
(Human, Taunusstein, Germany) and VIDAS. Concerning toxoplasma ELISA
(Human), IgM and IgG were positive for 5 of 19 and 27 of 27 sera,
respectively, while VIDAS was positive for 1 of 19 and 23 of 27 sera,
respectively. An arbitrator Toxo ELISA (Organon) for IgG and IgM
confirmed the VIDAS results. The sera with discrepant Human Toxo ELISA
IgG & IgM had O.D. readings close to the cut-off values. In addition to
the above noted characteristics, the VIDAS is a reliable instrument to
introduce in the clinical laboratories performing serodiagnosis of
infectious diseases.
DE Acquired Immunodeficiency Syndrome/*DIAGNOSIS Antibodies,
Protozoan/BLOOD Antibodies, Viral/BLOOD Comparative Study
Enzyme-Linked Immunosorbent Assay/*METHODS Human HIV Antibodies/BLOOD
HIV-1/*ISOLATION & PURIF HIV-2/*ISOLATION & PURIF IgG/BLOOD IgM/BLOOD
Rubella/*DIAGNOSIS Serodiagnosis/*METHODS Toxoplasmosis/*DIAGNOSIS
MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).